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Nitrofuran (AOZ) ELISA Test Kit

Shenzhen Biokau Sci-tech Co.,ltd

Nitrofuran (AOZ) ELISA Test Kit

Place of Origin : Shenzhen

Brand Name : Biokau

Model Number : BKU-10002

Certification : ISO9001

Price : 320USD

Packaging Details : 96T/kit

Delivery Time : 3 days

Payment Terms : TT% in advance

MOQ : 1

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Nitrofuran (AOZ) ELISA Test Kit

Catalog No. BKU-10002

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of AOZ in the sample. The coupling antigens are pre-coated on the micro-well stripes. The AOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AOZ in it. This value is compared to the standard curve and the AOZ concentration is subsequently obtained.


2. Technical specifications

Sensitivity: 0.02 ppb

Detection limit

Tissue(Chicken, duck, porcine meat, beef and mutton) 0.04 ppb

Liver(Chicken, duck, porcine meat, beef and mutton) 0.04 ppb

Honey, milk, intestine, urine 0.04 ppb

Fish, shrimp(some interference) 0.1 ppb

Recovery rate

Tissue, liver 90±10%

Honey, milk, intestine 75±15%

Cross-reaction rate

AOZ 100%

AHD <0.01%

AMOZ <0.01%

SEM <0.01%

3. Components

  • Micro-well strips: 12 strips with 8 removable wells each
  • 6× standard solution (1 mL each): 0 ppb, 0.02 ppb, 0.06 ppb, 0.18 ppb, 0.54 ppb and 1.62 ppb
  • Enzyme conjugate (7 mL) red cap
  • Antibody working solution (7 mL) blue cap
  • Substrate A solution (7 mL) white cap
  • Substrate B solution (7 mL) black cap
  • Stop solution (7 mL) yellow cap
  • 20× concentrated washing buffer (40 mL) white cap
  • 2× concentrated redissolving solution (50 mL) transparent cap
  • 2-Nitrobenzaldehyde (C7H5NO3)(10 mL) white cap

4. Materials required but not provided

  • Equipments: microplate reader, printer, homogenizer, nitrogen-drying device, vortex, centrifuge, measuring pipets, balance (a reciprocal sensibility of 0.01 g);
  • Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 250 µL;
  • Reagents: NaOH, ethyl acetate, n-Hexane, HCI (approx 36.5%), K2HPO4·3H2O(for all samples), K2Fe(CN)5NO·3H2O and ZnSO4·7H2O (for milk sample)


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Nitrofuran (AOZ) ELISA Test Kit

      

Food Safety

      

ELISA test

      
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