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Chloramphenicol(CAP) ELISA Test Kit

Shenzhen Biokau Sci-tech Co.,ltd

Chloramphenicol(CAP) ELISA Test Kit

Place of Origin : Shenzhen

Brand Name : Biokau

Model Number : BKU-10007

Certification : ISO9001

Price : 120USD

Packaging Details : 96T/kit

Delivery Time : 3 days

Payment Terms : TT% in advance

MOQ : 1

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Chloramphenicol ELISA test Kit

Catalog No. BKU-10007

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Chloramphenicol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Chloramphenicol antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chloramphenicol in it. This value is compared to the standard curve and the Chloramphenicol concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.05 ppb

Detection limit:

Intestine 0.1 ppb

Honey 0.15 ppb

Milk 0.05 ppb

Urine, serum 0.1 ppb

Chicken/liver, pork/liver, fish, shrimp 0.05 ppb

Recovery rate

Chicken/liver, pork/liver, fish, shrimp 80±10%

Honey 70±10%

Milk 85±15%

Cross-reaction rate:

Chloramphenicol 100%

Thiamphenicol < 0.1%

Florfeniol < 0.1%

3. Components

  • Micro-well strips: 12 strips with 8 removable wells each
  • 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb
  • Enzyme conjugate (12 mL) red cap
  • Concentrated antibody working solution (1 mL) blue cap
  • Substrate A solution (7 mL) white cap
  • Substrate B solution (7 mL) black cap
  • Stop solution (7 mL) yellow cap
  • 20× concentrated washing buffer (40 mL) white cap
  • 2× concentrated redissolving solution (50 mL) transparent cap

4. Materials required but not provided

  • Equipments: microplate reader, homogenizer, nitrogen-drying device, vortex, centrifuge, measuring pipets, and balance( a sensibility reciprocal of 0.01 g).
  • Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 250 µL.
  • Reagents: Ethyl acetate, Acetonitrile (CH3CN), N-hexane, Na2Fe(CN)5·NO·2H2O, ZnSO4.7H2O, Beta-Glucuronidase (Merck, Art. No. 4114), CH3COONa, CH3COOH

Product Tags:

Chloramphenicol(CAP) ELISA Test Kit


Food Safety


ELISA test

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