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Shenzhen Biokau Sci-tech Co.,ltd
Food safety ELISA test kit and animal disease rapid test kit--Biokau for a better life
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Florfenicol ELISA Test Kit

Shenzhen Biokau Sci-tech Co.,ltd

Florfenicol ELISA Test Kit

Place of Origin : Shenzhen

Brand Name : Biokau

Model Number : BKU-10008

Certification : ISO9001

Price : 500USD

Packaging Details : 96T/kit

Delivery Time : 3 days

Payment Terms : TT% in advance

MOQ : 1

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Florfenicol ELISA Test Kit

Catalog No. BKU-10008

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Florfenicol in the tissue (chicken, pork), fish, shrimp and honey. The coupling antigens are pre-coated on the micro-well stripes. The Florfenicol in the sample and the conjugate antigens pre-coated on the micro-well stripes compete for the anti-Florfenicol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value has a negative correlation with the Florfenicol concentration in the sample. This value is compared to the standard curve and the Florfenicol concentration is subsequently obtained.


2. Technical specifications

Sensitivity : 0.5 ppb

Detection limit:

Honey 1.5 ppb

Meat, liver, shrimp, fish 0.5 ppb

Recovery rate:

Honey 70 ±10%

Meat, liver, shrimp, fish 80 ±10%

Cross-reaction rate:

Florfenicol 100%

Florfenicol Amine 11%

Thiamphenicol < 0.1%

Chloramphenicol < 0.1%


3. Components

  • Micro-well strips: 12 strips with 8 removable wells each
  • 6× standard solution (1 mL each): 0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb and 40.5 ppb
  • Enzyme conjugate (12 mL) red cap
  • Concentrated Antibody working solution (1 mL) blue cap
  • Substrate A solution (7 mL) white cap
  • Substrate B solution (7 mL) black cap
  • Stop solution (7 mL) yellow cap
  • 20× concentrated washing buffer (40 mL) white cap
  • 2× concentrated redissolving solution (50 mL) transparent cap

4. Materials required but not provided

  • Equipments: microplate reader, printer, mixer or stomacher, nitrogen-drying device, oscillator, centrifuge, measuring pipets, balance( a reciprocal sensibility of 0.01 g)
  • Micropipettors: single-channel 20-200µL and 100-1000 µL, and multi-channel 250 µL;
  • Reagents: ethyl acetate, N-hexane


Product Tags:

Florfenicol ELISA Test Kit

      

Food Safety

      

ELISA test

      
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