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Shenzhen Biokau Sci-tech Co.,ltd
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Neomycin ELISA test kit

Shenzhen Biokau Sci-tech Co.,ltd

Neomycin ELISA test kit

Place of Origin : Shenzhen

Brand Name : Biokau

Model Number : BKU-10005

Certification : ISO9001

Price : 350USD

Packaging Details : 96T/kit

Delivery Time : 3 days

Payment Terms : TT% in advance

MOQ : 1

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Neomycin ELISA test Kit

Catalog No. BKU-10005

1. Principle

The Biokau® Neomycin ELISA test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Neomycin residues in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Neomycin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Neomycin in it. This value is compared to the standard curve and the Neomycin concentration is subsequently obtained.


2. Technical specifications

Sensitivity: 0.5 ppb

Detection limit

Honey 10 ppb

Chicken/liver, pork/liver, fish, shrimp 10 ppb

Recovery rate

Chicken, pork, honey, fish, shrimp 85±15%

Liver(chicken or porcine) 70±10%

Cross-reaction rate

Neomycin 100%

Streptomycin < 0.1%

Gentamicin < 0.1%

Dihydrostreptomycin < 0.1%

Kanamycin < 0.1%

Tobramycin < 0.1%

Sisomicin < 0.1%


3. Components

  • Micro-well strips: 12 strips with 8 removable wells each
  • 6× standard solution (1 mL each): 0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb and 40.5 ppb
  • Enzyme conjugate (7 mL) red cap
  • Antibody concentrated solution (7 mL) blue cap
  • Substrate A solution (7 mL) white cap
  • Substrate B solution (7 mL) black cap
  • Stop solution (7 mL) yellow cap
  • 20× concentrated washing buffer (40 mL) white cap
  • 2× concentrated redissolving solution (50 mL) transparent cap

4. Materials required but not provided

  • Equipments: microplate reader, printer, homogenizer, nitrogen-drying device, vortex, centrifuge, measuring pipets, and balance( a sensibility reciprocal of 0.01 g).
  • Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 250 µL.
  • Reagents: Trichloroacetic acid(TCA), NaOH, deionized water,

5. Sample pre-treatment

Instructions

The following points must be dealt with before the pre-treatment of any kind of sample:

  • Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents;
  • Before the experiment, each experimental equipment must be checked to be clean and should be re-cleaned if necessary, in order to avoid the contamination that interferes with the experimental results.

Solution preparation before sample pre-treatment

  • the 2×concentrated redissolving solution is mixed with deionized water at 1:1 (1 mL concentrated redissolbing solution + 1 mL deionized water).
  • 3% Trichloroacetic acid(for tissue sample): dissolve 3 g Trichloroacetic acid in the deionized water to 100 mL.
  • 2% Trichloroacetic acid(for honey sample): dissolve 2 g Trichloroacetic acid in the deionized water to 100 mL
  • 1 M NaOH: dissolve 4 g NaOH in the deionized water to 100 mL

5.1 Tissue(Shrimp, fish, meat, liver) sample

  • Take 2± 0.05 g of the homogenized sample into 50 mL tube, add 8 mL 3% Trichloroacetic acid, shake properly for 10 min, centrifuge at above 4000 r/min at room temperature (20-25℃) for 10 min.
  • Take the supernatant to another tube, adjust PH to 7.0-7.5 with NaOH, Dilute it with diluted redissolving solution at 1:4(100 µL sample+400µL diluted redissolving solution) .
  • Take 50 µL for analysis.

Fold of dilution of the sample: 20


5.2 Honey sample

  • Take 2± 0.05 g sample into 50 mL tube, add 8 mL 2% Trichloroacetic acid, shake properly for 10 min, centrifuge at above 4000 r/min at room temperature (25℃) for 10 min.
  • Take the supernatant to another tube, adjust PH to 7.0-7.5 with NaOH, Dilute it with diluted redissolving solution at 1:4(100 µL sample+400µL diluted redissolving solution) .
  • Take 50 µL for analysis.

Fold of dilution of the sample: 20


6. ELISA procedures

6.1 Instructions

  • Bring all reagents and micro-well strips to the room temperature (20-25 ℃) before use;
  • Return all reagents to 2-8 ℃ immediately after use;
  • The reproducibility of the ELISA analysis, to a large degree, depends on the consistency of plate washing. The correct operation of plate washing is the key point in ELISA the procedures;
  • For the incubation at constant temperatures, all the samples and reagents must avoid light exposure, and each microplate should be sealed by the cover membrane.


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