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Tetracyclines(TCs) ELISA Test Kit

Shenzhen Biokau Sci-tech Co.,ltd

Tetracyclines(TCs) ELISA Test Kit

Place of Origin : Shenzhen

Brand Name : Biokau

Model Number : BKU-10006

Certification : ISO9001

Price : 185USD

Packaging Details : 96T/kit

Delivery Time : 3 days

Payment Terms : TT% in advance

MOQ : 1

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Tetracyclines(TCs) ELISA Test Kit

Catalog No. BKU-10006

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Tetracyclines in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Tetracyclines in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Tetracyclines antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tetracyclines in it. This value is compared to the standard curve and the Tetracyclines concentration is subsequently obtained.


2. Technical specifications

Sensitivity: 0.05 ppb

Detection limit:

Chicken,duck, pork(meat or liver), shrimp, fish, milk

Tetracyclines 1ppb

Minocycline 1ppb

RoliTetracyclines 1ppb

Aureomycin 1ppb

Demethylchlor Tetracyclines 3ppb

Terramycin 2ppb

Doxycycline 3ppb


Honey

Tetracyclines 2ppb

Minocycline 2ppb

RoliTetracyclines 2ppb

Aureomycin 2ppb

Demethylchlor Tetracyclines 6ppb

Terramycin 4ppb

Doxycycline 6ppb

Cross-reaction rate:

Tetracyclines 100%

Minocycline 125%

RoliTetracyclines 110%

Aureomycin 100%

Demethylchlor Tetracyclines 35%

Terramycin 58%

Doxycycline 45%

Recovery rate:

Tissue, milk 85±10%

Honey 70±10%


3. Components

  • Micro-well strips: 12 strips with 8 removable wells each
  • 6× standard solution (1 mL each): 0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb, 40.5 ppb
  • Enzyme conjugate (12 mL) red cap
  • Antibody working solution (7 mL) blue cap
  • Substrate A solution (7 mL) white cap
  • Substrate B solution (7 mL) black cap
  • Stop solution (7 mL) yellow cap
  • 20× concentrated washing buffer (40 mL) white cap
  • 5× concentrated redissolving solution (50 mL) transparent cap

4. Materials required but not provided

  • Equipments: microplate reader (450 nm / 630 nm), homogenizer, oscillator, centrifuge, balance (a sensibility reciprocal of 0.01 g), measuring pipets
  • Micropipettors: single-channel 20 to 200 µL and 100 to 1000 µL, and multi-channel 250 µL;
  • Reagents: NaOH, Trichloroacetic acid, Na2HPO4·12H2O, deionized water, C6H8O7·H2O, EDTA·Na2·2H2O, H2C2O4, CH3OH(≥99.5%), RIDARC18 column


Product Tags:

Tetracyclines(TCs) ELISA Test Kit

      

Food Safety

      

ELISA test

      
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