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Shenzhen Biokau Sci-tech Co.,ltd
Food safety ELISA test kit and animal disease rapid test kit--Biokau for a better life
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Tylosin ELISA Test Kit

Shenzhen Biokau Sci-tech Co.,ltd

Tylosin ELISA Test Kit

Place of Origin : Shenzhen

Brand Name : Biokau

Model Number : BKU-10020

Certification : ISO9001

Price : 350USD

Packaging Details : 96T/kit

Delivery Time : 3 days

Payment Terms : TT% in advance

MOQ : 1

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Tylosin ELISA Test Kit

Catalog No. BKU-10020

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Tylosin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Tylosin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Tylosin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tylosin in it. This value is compared to the standard curve and the Tylosin concentration is subsequently obtained.


2. Technical specifications

Sensitivity: 1.5 ppb

Detection limit

Meat, liver 3 ppb

Honey 1.5 ppb

Recovery rate

Meat 90±15%

Liver 80±10%

Honey 85±15%


3. Components

  • Micro-well strips: 12 strips with 8 removable wells each
  • 6× standard solution (1 mL each): 0 ppb, 1.5 ppb, 4.5 ppb, 13.5p pb ,40.5 ppb,121.5 ppb
  • Enzyme conjugate (7 mL) red cap
  • Antibody working solution (7 mL) blue cap
  • Substrate A solution (7 mL) white cap
  • Substrate B solution (7 mL) black cap
  • Stop solution (7 mL) yellow cap
  • 20× concentrated washing buffer (40 mL) white cap
  • 2× concentrated redissolving solution (50 mL) transparent cap

4. Materials required but not provided

  • Equipments: microplate reader (450 nm / 630 nm), homogenizer, oscillator, votex, centrifuge, and balance (a sensibility reciprocal of 0.01 g)
  • Micropipettors: single-channel 20 to 200 µL and 100 to 1000 µL, and multi-channel 250 µL;
  • Reagents: ethanenitrile (CH3CN), Methanol, deionized water, 0.1 M HCI, NaOH, CHCl3


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Tylosin ELISA Test Kit

      

Food Safety

      

Tylosin

      
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