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Shenzhen Biokau Sci-tech Co.,ltd
Food safety ELISA test kit and animal disease rapid test kit--Biokau for a better life
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Sulfanethazine (SM2) ELISA Test Kit

Shenzhen Biokau Sci-tech Co.,ltd

Sulfanethazine (SM2) ELISA Test Kit

Place of Origin : Shenzhen

Brand Name : Biokau

Model Number : BKU-10010

Certification : ISO9001

Price : 220USD

Packaging Details : 96T/kit

Delivery Time : 3 days

Payment Terms : TT% in advance

MOQ : 1

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Sulfadiazine ELISA Test Kit

1. Principle

The test kit is based on the competitive enzyme immunoassay for the detection of Sulfadiazine in the chicken, pork, milk, honey and egg. The coupling antigens are pre-coated on the micro-well stripes. The Sulfamethazine in the sample and the conjugated antigen pre-coated on the micro-well stripes compete for the anti-Sulfamethazine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Sulfamethazine concentration in the sample. This value is compared to the standard curve and the Sulfamethazine concentration is subsequently obtained.


2. Technical specifications

Sensitivity : 0.4 ppb

Detection limit:

Meat(chicken, pork), liver(chicken, pork), honey, egg 0.4 ppb

Serum, urine 1.6 ppb

Milk 8 ppb

Recovery rate:

Meat(chicken, pork), liver(chicken, pork) 90 ±10%

Egg 65 ±10%

Milk, honey, serum 70 ±10%

Cross-reaction rate:

Sulfadiazine (SD or SDZ) 100%

Sulfamonomethoxine (SMM) 7%

Sulfamerazine (SM1) 12%

Sulfadimethoxypyrimidine (SDM) 12.5%


3. Components

  • Micro-well strips: 12 strips with 8 removable wells each
  • 6× standard solution (1 mL each): 0 ppb, 0.4 ppb, 1.2 ppb, 3.6 ppb, 10.8 ppb and 32.4 ppb
  • Enzyme conjugate (7 mL) red cap
  • Antibody working solution (10 mL) blue cap
  • Substrate A solution (7 mL) white cap
  • Substrate B solution (7 mL) black cap
  • Stop solution (7 mL) yellow cap
  • 20× concentrated washing buffer (40 mL) white cap
  • 2× concentrated redissolving solution (50 mL) transparent cap

4. Materials required but not provided

  • Equipments: microplate reader, printer, mixer or stomacher, nitrogen-drying device, oscillator, centrifuge, measuring pipets, balance( a reciprocal sensibility of 0.01 g);
  • Micropipettors: single-channel 20-200 µL and 100-1000 µL, and multi-channel 250 µL;

3) Reagents: ethyl acetate, N-hexane, Na2HPO4·12H2O, NaH2PO4·2H2O, HCl (conc.), NaOH



Product Tags:

Sulfanethazine (SM2) ELISA Test Kit

      

Food Safety

      

SM2

      
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