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Classical Swine Fever Virus Antibody ELISA Test Kit (CSFV Ab)

Shenzhen Biokau Sci-tech Co.,ltd

Classical Swine Fever Virus Antibody ELISA Test Kit (CSFV Ab)

Place of Origin : Shenzhen

Brand Name : Biokau

Price : 220 USD/kit

Delivery Time : 3 days

Payment Terms : TT

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1. Brief

The Biokau® hog cholera virus antibody test kit, or called Classical Swine Fever Virus Antibody ELISA Test Kit (CSFV Ab), is used for the detection of hog cholera virus antibodies in porcine serum; assessment of immunity conditions against hog cholera virus in the pig farms and epidemiology investigation of the hog cholera.

2. Principle

The Biokau® hog cholera virus antibody ELISA test kit is made from the antigen coated microtiter plate, goat-anti-pig IgG-HRP and other reagents. It applies the indirect ELISA principle to test the antibodies against hog cholera virus in porcine serum. In the test, the coated antigen combine with CSFV-IgG in serum, then add IgG-HRP to specifically bind with complex of antibody-antigens on the microplate. With the TMB substrate, it will generate an amount of color. The depth of color is relative with the content of the CSFV-IgG, when the value of color is greater than the cut-off value, the pigs are vaccinated well.

3. Components

1 CSFV coated microplate 2 pieces (96 wells)

2 CSFV Negative serum(green lid) 1 tube (1 mL)

3 CSFV Positive serum (red lid) 1 tube (1 mL)

4 CSFV Enzyme conjugate (yellow lid) 1 bottle (22 mL)

5 20×Wash Concentrate (white lid) 1 bottle (50 mL)

6 Substrate A (red lid)/B (black lid) 1 bottle each (12 mL/bottle)

7 Stop solution(blue lid) 1 bottle (12 mL)

8 Sample diluent solution(transparent lid) 1 bottle (25 mL)

9 Adhesive Foil 6 pieces

10 Instruction 1 piece

4. Material Required But not Provided

  • Microplate Reader(wave length: 450/630 nm).
  • Microplate Washer.
  • Micropipettes, adjustable.
  • Constant temperature box or incubator.
  • Oscillator.

5. Sample requirement

  • The samples are porcine serum, which should be collected with no bacteria. The storage time should be less than 1 week at 2-8 ℃, if for long term, it should be kept at -20℃.
  • Avoid to use the samples with severe hemolysis, precipitate, contaminated by bacteria or protein suspension.
  • The EDTA, heparin sodiun and other anticoagulants will not affect the results.

6. Procedure

  • Take out the coated plates(Can be detached) and record the sample position on a worksheet. Set one blank control well, add nothing. Set 2 wells for negative control serum and 2 wells for positive control serum, 100 μL/well. Others are wells for samples, add 100μL/well of Sample diluent solution, then add 10 μL serum sample seperately.
  • Mix gently, incubate at 37℃ for 30 min.
  • Remove adhesive foil. Pour the liquid out of the wells, add Washing solution(dillute the Wash Concentrate at 1:19 with distilled water) into each well, 300 μL/well, stand for 1 min. Repeat 5 times, at last time pat to dry on absorbent paper.
  • Add 100 μL enzyme comjugate into each well(except the blank well).
  • Cover plate with new adhesive foil. Incubate at 37 ℃ for 30 min.
  • Repeat step 3(washing).
  • Add substrate A one drop (50 μL) and substrate B one drop (50 μL) into each well, mix properly, incubate for 15 min at 37 ℃ in the dark with new adhesive foil.
  • Add stop solution one drop (50 μL) into each well, mix gently and determine the result within 5-30 min.
  • Measure the optical density(OD) with a photometer at 450 nm(Reference-wavelength: 630 nm). Set zero for the blank well, and read the OD 450 value of samples on the Microplate reader.

7. ELISA analysis

For the assay to be valid the following specifications must be met. The CSFV-Positive control mean (PC:OD450) must be greater than 0.70, the CSFV-Negative control mean must be less than 0.05.

Cut-Off Value (C.O.) = 0.2×PC:OD450. The presence or absence of antibody to CSFV is determined by calculating the sample to C.O. ratio.

If the S/C.O. ≥1, the sample is classified as POSITIVE for CSFV antibodies;

If the S/C.O. <1, the sample is classified as NEGATIVE for CSFV antibodies.

8. Specification

Packing : 96 wells×2.

Expiry date: 12 months.

Storage: Storing at 2~8 ℃ in the dark, not freeze.

Product Tags:

Hog Cholera Virus




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